// Qualitative Detection & Quantitative Determination of Traces of Allergens
// Detection Packages According to Directive (EU) 1169/2011
According to the European Food Information Regulation EU 1169-2011, substances or products that may cause allergies or intolerances must be clearly identified and highlighted in the list of ingredients. Contaminants that might accidentally enter products (depending on transport or storage conditions and manufacturing processes) are also relevant for manufacturers and consumers. In order to meet both legal requirements and consumer demands, raw materials as well as semi-finished and finished products are tested for allergenic components in the course of quality controls. The variety of sample matrices and process steps influence the choice of suitable detection methods. The CONGEN ServiceLab is a specialist in the field of allergen analytics using real-time PCR. Specific and sensitive methods enable rapid analysis with reproducible results.
Overview Allergen Analyses
- Brazil Nut
- Gluten-containing Cereals
The analysis comprises the following steps:
Homogenization and DNA Extraction
You send a representative part of the sample material to our service laboratory. The sample is homogenized, and the DNA is extracted in duplicate. Of course, appropriate controls will be carried out:
- The negative extraction control as an indication of buffer contamination or carry over during extraction.
- The positive extraction control (as proof of the functionality of the method used) is carried out in the form of a lot-dependent process control.
The extracted sample DNA can be analysed for up to six parameters using real-time PCR. In qualitative procedures, negative, inhibition, and positive controls are routinely used. If the controls show the expected results, you will receive a test report with the corresponding results and the system-specific detection limit. Please note that the determination is not matrix-specific.
Qualitative DNA Detection / Screening
The detection of allergenic components is designed for the determination of contaminants or hidden allergens. The detection limit of the methods is therefore in the lower mg/kg range.
Quantitative DNA Detection
If a sample has been tested qualitatively positive for one or more allergenic components or if expected allergenic components are to be investigated, they can be quantified using real-time PCR. The quantification of allergenic components also takes place in ranges below 0.1% and is not designed for determining the content of ingredients in the % range.
The content of allergen constituents in samples is determined using a standard series and reference material (with a defined concentration). The sample matrix and processing influence the quantitative result as well as the detection or determination limit. A sample-specific detection limit is not determined.
We carry out allergen quantification using a standard. This is a reference material based on maize flour doped with 40 mg of allergenic ingredient/kg maize flour. For liquids, samples with a high fat content, swabs, sponges, and comparable swab samples, a quantitative statement is generally not possible.
Protein ELISA and Enzymatics
In addition, we offer alternative methods such as protein ELISA or enzymatic detection for analytes that cannot be determined by real-time PCR. Extraction and analysis are carried out according to the manufacturer’s specifications. Here too, no quantitative statement is possible for swabs, sponges, and comparable swab samples.
Overview Protein ELISA and Enzymatics
- Hen´s egg protein
- Total Milk